Efficient Knockdown and Lack of Passenger Strand Activity by Dicer-Independent shRNAs Expressed from Pol II-Driven MicroRNA Scaffolds

• Hoofdauteurs: Erik Kaadt (Auteur), Sidsel Alsing (Auteur), Claudia R. Cecchi (Auteur), Christian Kroun Damgaard (Auteur), Thomas J. Corydon (Auteur), Lars Aagaard (Auteur)
• Formaat: Boek
• Gepubliceerd in: Elsevier, 2019-03-01T00:00:00Z.
• Onderwerpen: article
• Online toegang: Connect to this object online.

The expression of short hairpin RNAs (shRNAs) may result in unwanted activity from the co-processed passenger strand. Recent studies have shown that shortening the stem of conventional shRNAs abolishes passenger strand release. These Dicer-independent shRNAs, expressed from RNA polymerase III (Pol I...