Efficient Knockdown and Lack of Passenger Strand Activity by Dicer-Independent shRNAs Expressed from Pol II-Driven MicroRNA Scaffolds

• 主要な著者: Erik Kaadt (著者), Sidsel Alsing (著者), Claudia R. Cecchi (著者), Christian Kroun Damgaard (著者), Thomas J. Corydon (著者), Lars Aagaard (著者)
• フォーマット: 図書
• 出版事項: Elsevier, 2019-03-01T00:00:00Z.
• 主題: article
• オンライン･アクセス: Connect to this object online.

The expression of short hairpin RNAs (shRNAs) may result in unwanted activity from the co-processed passenger strand. Recent studies have shown that shortening the stem of conventional shRNAs abolishes passenger strand release. These Dicer-independent shRNAs, expressed from RNA polymerase III (Pol I...