Generation of a multipurpose Prdm16 mouse allele by targeted gene trapping

• Main Authors: Alexander Strassman (Author), Frank Schnütgen (Author), Qi Dai (Author), Jennifer C. Jones (Author), Angela C. Gomez (Author), Lenore Pitstick (Author), Nathan E. Holton (Author), Russell Moskal (Author), Erin R. Leslie (Author), Harald von Melchner (Author), David R. Beier (Author), Bryan C. Bjork (Author)
• Format: Book
• Published: The Company of Biologists, 2017-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Gene trap mutagenesis is a powerful tool to create loss-of-function mutations in mice and other model organisms. Modifications of traditional gene trap cassettes, including addition of conditional features in the form of Flip-excision (FlEx) arrays to enable directional gene trap cassette inversions...