CRISPR Start-Loss: A Novel and Practical Alternative for Gene Silencing through Base-Editing-Induced Start Codon Mutations

• Main Authors: Siyu Chen (Author), Wanhua Xie (Author), Zhiquan Liu (Author), Huanhuan Shan (Author), Mao Chen (Author), Yuning Song (Author), Hao Yu (Author), Liangxue Lai (Author), Zhanjun Li (Author)
• Format: Book
• Published: Elsevier, 2020-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

CRISPR-Cas9-mediated gene knockout and base-editing-associated induction of STOP codons (iSTOP) have been widely used to exterminate the function of a coding gene, while they have been reported to exhibit side effects. In this study, we propose a novel and practical alternative method referred to as...