Modification of PCR Conditions and Design of Exon-Specific Primers for the Efficient Molecular Diagnosis of PKD1 Mutations

• Main Authors: WeiQiang Liu (Author), Min Chen (Author), Jun Wei (Author), WenYin He (Author), ZhiHua Li (Author), XiaoFang Sun (Author), YuLing Shi (Author)
• Format: Book
• Published: Karger Publishers, 2014-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background/Aims: Autosomal-dominant polycystic kidney disease (ADPKD) is a heterogeneous genetic disorder caused by mutations in the PKD1 and PKD2 genes. Currently, long-range PCR followed by nested PCR and sequencing (LRNS) is the gold standard approach for PKD1 testing. However, LRNS is complicate...