Modification of PCR Conditions and Design of Exon-Specific Primers for the Efficient Molecular Diagnosis of PKD1 Mutations

• Główni autorzy: WeiQiang Liu (Autor), Min Chen (Autor), Jun Wei (Autor), WenYin He (Autor), ZhiHua Li (Autor), XiaoFang Sun (Autor), YuLing Shi (Autor)
• Format: Książka
• Wydane: Karger Publishers, 2014-11-01T00:00:00Z.
• Hasła przedmiotowe: article
• Dostęp online: Connect to this object online.

Background/Aims: Autosomal-dominant polycystic kidney disease (ADPKD) is a heterogeneous genetic disorder caused by mutations in the PKD1 and PKD2 genes. Currently, long-range PCR followed by nested PCR and sequencing (LRNS) is the gold standard approach for PKD1 testing. However, LRNS is complicate...