Modification of PCR Conditions and Design of Exon-Specific Primers for the Efficient Molecular Diagnosis of PKD1 Mutations

• Ngā kaituhi matua: WeiQiang Liu (Author), Min Chen (Author), Jun Wei (Author), WenYin He (Author), ZhiHua Li (Author), XiaoFang Sun (Author), YuLing Shi (Author)
• Hōputu: Pukapuka
• I whakaputaina: Karger Publishers, 2014-11-01T00:00:00Z.
• Ngā marau: article
• Urunga tuihono: Connect to this object online.

Background/Aims: Autosomal-dominant polycystic kidney disease (ADPKD) is a heterogeneous genetic disorder caused by mutations in the PKD1 and PKD2 genes. Currently, long-range PCR followed by nested PCR and sequencing (LRNS) is the gold standard approach for PKD1 testing. However, LRNS is complicate...