Effects of Decomplexation Rates on Ternary Gene Complex Transfection with <i>α</i>-Poly(<span style="font-variant: small-caps">l</span>-Lysine) or <i>ε</i>-Poly(<span style="font-variant: small-caps">l</span>-Lysine) as a Decomplexation Controller in An Easy-To-Transfect Cell or A Hard-To-Transfect Cell

• Main Authors: Kyoungnam Kim (Author), Kitae Ryu (Author), Hana Cho (Author), Min Suk Shim (Author), Yong-Yeon Cho (Author), Joo Young Lee (Author), Hye Suk Lee (Author), Han Chang Kang (Author)
• Format: Book
• Published: MDPI AG, 2020-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

The tight binding of pDNA with a cationic polymer is the crucial requirement that prevents DNA degradation from undesired DNase attack to safely deliver the pDNA to its target site. However, cationic polymer-mediated strong gene holding limits pDNA dissociation from the gene complex, resulting in a...