A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging

• Main Authors: Yao eChen (Author), Jessica Lizette Saulnier (Author), Gary eYellen (Author), Bernardo eSabatini (Author)
• Format: Book
• Published: Frontiers Media S.A., 2014-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Neuromodulators have profound effects on behavior, but the dynamics of their intracellular effectors has remained unclear. Most neuromodulators exert their function via G-protein-coupled receptors (GPCRs). One major challenge for understanding neuromodulator action is the lack of dynamic readouts of...