A CD3/CD28 microbead-based HIV-1 viral outgrowth assay

• Main Authors: Yury V. Kuzmichev (Author), Rebecca T. Veenhuis (Author), Christopher W. Pohlmeyer (Author), Caroline C. Garliss (Author), Victoria E.K. Walker-Sperling (Author), Joel N. Blankson (Author)
• Format: Book
• Published: Elsevier, 2017-04-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Aims: Latently infected resting CD4 T cells represent a major barrier to HIV-1 eradication efforts. The standard assays used for measuring this reservoir induce activation of resting CD4 T cells with either phytohaemagglutinin (PHA) with irradiated feeder cells, or with anti-CD3 antibodies. We desig...