A CD3/CD28 microbead-based HIV-1 viral outgrowth assay

• Autori principali: Yury V. Kuzmichev (Autore), Rebecca T. Veenhuis (Autore), Christopher W. Pohlmeyer (Autore), Caroline C. Garliss (Autore), Victoria E.K. Walker-Sperling (Autore), Joel N. Blankson (Autore)
• Natura: Libro
• Pubblicazione: Elsevier, 2017-04-01T00:00:00Z.
• Soggetti: article
• Accesso online: Connect to this object online.

Aims: Latently infected resting CD4 T cells represent a major barrier to HIV-1 eradication efforts. The standard assays used for measuring this reservoir induce activation of resting CD4 T cells with either phytohaemagglutinin (PHA) with irradiated feeder cells, or with anti-CD3 antibodies. We desig...