Rapid and sensitive point-of-care detection of Leptospira by RPA-CRISPR/Cas12a targeting lipL32.

• Main Authors: Sirawit Jirawannaporn (Author), Umaporn Limothai (Author), Sasipha Tachaboon (Author), Janejira Dinhuzen (Author), Patcharakorn Kiatamornrak (Author), Watchadaporn Chaisuriyong (Author), Jom Bhumitrakul (Author), Oraphan Mayuramart (Author), Sunchai Payungporn (Author), Nattachai Srisawat (Author)
• Format: Book
• Published: Public Library of Science (PLoS), 2022-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

<h4>Background</h4>One of the key barriers preventing rapid diagnosis of leptospirosis is the lack of available sensitive point-of-care testing. This study aimed to develop and validate a clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 12a (CRI...