Mapping conformational changes on bispecific antigen-binding biotherapeutic by covalent labeling and mass spectrometry

• Päätekijät: Arnik Shah (Tekijä), Dipanwita Batabyal (Tekijä), Dayong Qiu (Tekijä), Weidong Cui (Tekijä), John Harrahy (Tekijä), Alexander R. Ivanov (Tekijä)
• Aineistotyyppi: Kirja
• Julkaistu: Elsevier, 2024-08-01T00:00:00Z.
• Aiheet: article
• Linkit: Connect to this object online.

Biotherapeutic's higher order structure (HOS) is a critical determinant of its functional properties and conformational relevance. Here, we evaluated two covalent labeling methods: diethylpyrocarbonate (DEPC)-labeling and fast photooxidation of proteins (FPOP), in conjunction with mass spectrom...