Mapping conformational changes on bispecific antigen-binding biotherapeutic by covalent labeling and mass spectrometry

• Main Authors: Arnik Shah (Author), Dipanwita Batabyal (Author), Dayong Qiu (Author), Weidong Cui (Author), John Harrahy (Author), Alexander R. Ivanov (Author)
• Format: Book
• Published: Elsevier, 2024-08-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Biotherapeutic's higher order structure (HOS) is a critical determinant of its functional properties and conformational relevance. Here, we evaluated two covalent labeling methods: diethylpyrocarbonate (DEPC)-labeling and fast photooxidation of proteins (FPOP), in conjunction with mass spectrom...