Mapping conformational changes on bispecific antigen-binding biotherapeutic by covalent labeling and mass spectrometry

• Hlavní autoři: Arnik Shah (Autor), Dipanwita Batabyal (Autor), Dayong Qiu (Autor), Weidong Cui (Autor), John Harrahy (Autor), Alexander R. Ivanov (Autor)
• Médium: Kniha
• Vydáno: Elsevier, 2024-08-01T00:00:00Z.
• Témata: article
• On-line přístup: Connect to this object online.

Biotherapeutic's higher order structure (HOS) is a critical determinant of its functional properties and conformational relevance. Here, we evaluated two covalent labeling methods: diethylpyrocarbonate (DEPC)-labeling and fast photooxidation of proteins (FPOP), in conjunction with mass spectrom...