Construction of keratin 5-IRES-eGFP knockin hESCs cell line

• Główni autorzy: Wen ZHENG (Autor), Yadan ZHONG (Autor), Xuan WANG (Autor), Huiting LIU (Autor), Bin YANG (Autor), Jun LIU (Autor)
• Format: Książka
• Wydane: editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022-08-01T00:00:00Z.
• Hasła przedmiotowe: article
• Dostęp online: Connect to this object online.

Objective To establish keratin 5-IRES-eGFP knockin hESCs cell line by using CRISPR/Cas9 technique, which could provide a cell model for the study of skin diseases with abnormal basal keratinocytes in the epidermis. Methods The sgRNA plasmid and target Donor plasmid targeting gene keratin 5 were cons...