Construction of keratin 5-IRES-eGFP knockin hESCs cell line

• Váldodahkkit: Wen ZHENG (Dahkki), Yadan ZHONG (Dahkki), Xuan WANG (Dahkki), Huiting LIU (Dahkki), Bin YANG (Dahkki), Jun LIU (Dahkki)
• Materiálatiipa: Girji
• Almmustuhtton: editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology, 2022-08-01T00:00:00Z.
• Fáttát: article
• Liŋkkat: Connect to this object online.

Objective To establish keratin 5-IRES-eGFP knockin hESCs cell line by using CRISPR/Cas9 technique, which could provide a cell model for the study of skin diseases with abnormal basal keratinocytes in the epidermis. Methods The sgRNA plasmid and target Donor plasmid targeting gene keratin 5 were cons...