Construction of keratin 5-IRES-eGFP knockin hESCs cell line
Objective To establish keratin 5-IRES-eGFP knockin hESCs cell line by using CRISPR/Cas9 technique, which could provide a cell model for the study of skin diseases with abnormal basal keratinocytes in the epidermis. Methods The sgRNA plasmid and target Donor plasmid targeting gene keratin 5 were cons...
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editoiral office of Journal of Diagnosis and Therapy on Dermato-venereology,
2022-08-01T00:00:00Z.
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