Compatibility and Fidelity of Mirror-Image Thymidine in Transcription Events by T7 RNA Polymerase

• Main Authors: Qingju Liu (Author), Yongqi Ke (Author), Yuhe Kan (Author), Xinjing Tang (Author), Xiangjun Li (Author), Yujian He (Author), Li Wu (Author)
• Format: Book
• Published: Elsevier, 2020-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Due to highly enzymatic d-stereoselectivity, l-nucleotides (l-2'-deoxynucleoside 5'-triphosphates [l-dNTPs]) are not natural targets of polymerases. In this study, we synthesized series of l-thymidine (l-T)-modified DNA strands and evaluated the processivity of nucleotide incorporation for...