Compatibility and Fidelity of Mirror-Image Thymidine in Transcription Events by T7 RNA Polymerase

• Hoofdauteurs: Qingju Liu (Auteur), Yongqi Ke (Auteur), Yuhe Kan (Auteur), Xinjing Tang (Auteur), Xiangjun Li (Auteur), Yujian He (Auteur), Li Wu (Auteur)
• Formaat: Boek
• Gepubliceerd in: Elsevier, 2020-09-01T00:00:00Z.
• Onderwerpen: article
• Online toegang: Connect to this object online.

Due to highly enzymatic d-stereoselectivity, l-nucleotides (l-2'-deoxynucleoside 5'-triphosphates [l-dNTPs]) are not natural targets of polymerases. In this study, we synthesized series of l-thymidine (l-T)-modified DNA strands and evaluated the processivity of nucleotide incorporation for...