CRISPR Interference-Mediated Silencing of the <i>mmpL3</i> Gene in <i>Mycobacterium smegmatis</i> and Its Impact on Antimicrobial Susceptibility

• Main Authors: Yonita Yuliani (Author), Azizah Fitriana Nurul Ilmi (Author), Suthidee Petsong (Author), Ajcharaporn Sawatpanich (Author), Sunisa Chirakul (Author), Tanittha Chatsuwan (Author), Tanapat Palaga (Author), Suwatchareeporn Rotcheewaphan (Author)
• Format: Book
• Published: MDPI AG, 2024-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Background: The discovery of novel therapeutic agents, especially those targeting mycobacterial membrane protein large 3 (<i>mmpL3</i>), has shown promise. In this study, the CRISPR interference-<i>Streptococcus thermophilus</i> nuclease-deactivated Cas9 (CRISPRi-dCas9<sub...