LAL test and RPT for endotoxin detection of CPT-11/DSPE-mPEG2000 nanoformulation: What if traditional methods are not applicable?

• Main Authors: Yanan Jin (Author), Juanjuan Jia (Author), Chan Li (Author), Jianqi Xue (Author), Jiabei Sun (Author), Kaiyuan Wang (Author), Yaling Gan (Author), Jing Xu (Author), Yaqin Shi (Author), Xingjie Liang (Author)
• Format: Book
• Published: Elsevier, 2018-05-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Endotoxin detection is an important step in drug characterization. Herein we found that a chemotherapeutic drug nanoformulation composed of irinotecan hydrochloride (CPT-11) and an amphiphilic molecule DSPE-mPEG2000 can interfere with the limulus amebocyte lysate assay (LAL). Furthermore, the rabbit...