Optimization of post-insertion method to conjugate Doxil with anti-CD133 monoclonal antibodies: Investigating the specific binding and cytotoxicity to colorectal cancer cells in vitro

• Main Authors: Karim Dadashi Noshahr (Author), Fahimeh Shamsi (Author), Peter Valtchev (Author), Parviz Kokhaei (Author), Maral Hemati (Author), Mohammad Reza Akbari Eidgahi (Author), Ali Khaleghian (Author)
• Format: Book
• Published: Elsevier, 2020-11-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

In this paper, Doxil coupled with anti-CD133 monoclonal antibodies made by either routine or optimized post-insertion technique, were compared with respect to their size, drug leakage, release pattern and the number of antibodies conjugated per single liposome. The results demonstrated that the numb...