Optimization of post-insertion method to conjugate Doxil with anti-CD133 monoclonal antibodies: Investigating the specific binding and cytotoxicity to colorectal cancer cells in vitro

• Egile Nagusiak: Karim Dadashi Noshahr (Egilea), Fahimeh Shamsi (Egilea), Peter Valtchev (Egilea), Parviz Kokhaei (Egilea), Maral Hemati (Egilea), Mohammad Reza Akbari Eidgahi (Egilea), Ali Khaleghian (Egilea)
• Formatua: Liburua
• Argitaratua: Elsevier, 2020-11-01T00:00:00Z.
• Gaiak: article
• Sarrera elektronikoa: Connect to this object online.

In this paper, Doxil coupled with anti-CD133 monoclonal antibodies made by either routine or optimized post-insertion technique, were compared with respect to their size, drug leakage, release pattern and the number of antibodies conjugated per single liposome. The results demonstrated that the numb...