The application of 2-NBDG as a fluorescent tracer for assessing hepatic glucose production in mice during hyperinsulinemic euglycemic clamp

• Principais autores: Shuainan Liu (Autor), Quan Liu (Autor), Sujuan Sun (Autor), Qian Jiang (Autor), Jun Peng (Autor), Zhufang Shen (Autor)
• Formato: Livro
• Publicado em: Elsevier, 2012-08-01T00:00:00Z.
• Assuntos: article
• Acesso em linha: Connect to this object online.

Methods of performing insulin clamps vary between laboratories. Here we present a protocol of insulin clamping in conscious mice, with the significant advantage of avoiding multiple surgical catheterizations and non-physiologic metabolism during the induction of anesthesia. Using this technique we a...