The application of 2-NBDG as a fluorescent tracer for assessing hepatic glucose production in mice during hyperinsulinemic euglycemic clamp

• Ngā kaituhi matua: Shuainan Liu (Author), Quan Liu (Author), Sujuan Sun (Author), Qian Jiang (Author), Jun Peng (Author), Zhufang Shen (Author)
• Hōputu: Pukapuka
• I whakaputaina: Elsevier, 2012-08-01T00:00:00Z.
• Ngā marau: article
• Urunga tuihono: Connect to this object online.

Methods of performing insulin clamps vary between laboratories. Here we present a protocol of insulin clamping in conscious mice, with the significant advantage of avoiding multiple surgical catheterizations and non-physiologic metabolism during the induction of anesthesia. Using this technique we a...