Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling

• Váldodahkkit: Yonggang Zhang (Dahkki), Gustavo Arango (Dahkki), Fang Li (Dahkki), Xiao Xiao (Dahkki), Raj Putatunda (Dahkki), Jun Yu (Dahkki), Xiao-Feng Yang (Dahkki), Hong Wang (Dahkki), Layne T. Watson (Dahkki), Liqing Zhang (Dahkki), Wenhui Hu (Dahkki)
• Materiálatiipa: Girji
• Almmustuhtton: BMC, 2018-09-01T00:00:00Z.
• Fáttát: article
• Liŋkkat: Connect to this object online.

Abstract Background CRISPR/CAS9 (epi)genome editing revolutionized the field of gene and cell therapy. Our previous study demonstrated that a rapid and robust reactivation of the HIV latent reservoir by a catalytically-deficient Cas9 (dCas9)-synergistic activation mediator (SAM) via HIV long termina...