Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling

• Main Authors: Yonggang Zhang (Author), Gustavo Arango (Author), Fang Li (Author), Xiao Xiao (Author), Raj Putatunda (Author), Jun Yu (Author), Xiao-Feng Yang (Author), Hong Wang (Author), Layne T. Watson (Author), Liqing Zhang (Author), Wenhui Hu (Author)
• Format: Book
• Published: BMC, 2018-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Abstract Background CRISPR/CAS9 (epi)genome editing revolutionized the field of gene and cell therapy. Our previous study demonstrated that a rapid and robust reactivation of the HIV latent reservoir by a catalytically-deficient Cas9 (dCas9)-synergistic activation mediator (SAM) via HIV long termina...