Rapid, Efficient, and Universally Applicable Genetic Engineering of Intestinal Organoid with a Sequential Monolayer to Three-Dimensional Strategy

• Hauptverfasser: Weili Han (Verfasst von), Guofang Lu (Verfasst von), Sheng Zhao (Verfasst von), Rui Wang (Verfasst von), Haohao Zhang (Verfasst von), Kun Liu (Verfasst von), Yongzhan Nie (Verfasst von), Jiaqiang Dong (Verfasst von)
• Format: Buch
• Veröffentlicht: Hindawi Limited, 2024-01-01T00:00:00Z.
• Schlagworte: article
• Online-Zugang: Connect to this object online.

Genetically modified intestinal organoids are being explored as potential surrogates of immortalized cell lines and gene-engineered animals. However, genetic manipulation of intestinal organoids is time-consuming, and the efficiency is far beyond satisfactory. To ensure the yield of the genetically...