Rapid, Efficient, and Universally Applicable Genetic Engineering of Intestinal Organoid with a Sequential Monolayer to Three-Dimensional Strategy

• Main Authors: Weili Han (Author), Guofang Lu (Author), Sheng Zhao (Author), Rui Wang (Author), Haohao Zhang (Author), Kun Liu (Author), Yongzhan Nie (Author), Jiaqiang Dong (Author)
• Format: Book
• Published: Hindawi Limited, 2024-01-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Genetically modified intestinal organoids are being explored as potential surrogates of immortalized cell lines and gene-engineered animals. However, genetic manipulation of intestinal organoids is time-consuming, and the efficiency is far beyond satisfactory. To ensure the yield of the genetically...