Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice

• প্রধান লেখক: Sara X. Chen (লেখক), Anna B. Osipovich (লেখক), Alessandro Ustione (লেখক), Leah A. Potter (লেখক), Susan Hipkens (লেখক), Rama Gangula (লেখক), Weiping Yuan (লেখক), David W. Piston (লেখক), Mark A. Magnuson (লেখক)
• বিন্যাস: গ্রন্থ
• প্রকাশিত: The Company of Biologists, 2011-07-01T00:00:00Z.
• বিষয়গুলি: article
• অনলাইন ব্যবহার করুন: Connect to this object online.

SUMMARY Fluorescent proteins (FPs) have great utility in identifying specific cell populations and in studying cellular dynamics in the mouse. To quantify the factors that determine both the expression and relative brightness of FPs in mouse embryonic stem cells (mESCs) and in mice, we generated eig...