Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice

• Main Authors: Sara X. Chen (Author), Anna B. Osipovich (Author), Alessandro Ustione (Author), Leah A. Potter (Author), Susan Hipkens (Author), Rama Gangula (Author), Weiping Yuan (Author), David W. Piston (Author), Mark A. Magnuson (Author)
• Format: Book
• Published: The Company of Biologists, 2011-07-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

SUMMARY Fluorescent proteins (FPs) have great utility in identifying specific cell populations and in studying cellular dynamics in the mouse. To quantify the factors that determine both the expression and relative brightness of FPs in mouse embryonic stem cells (mESCs) and in mice, we generated eig...