Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice

• Ngā kaituhi matua: Sara X. Chen (Author), Anna B. Osipovich (Author), Alessandro Ustione (Author), Leah A. Potter (Author), Susan Hipkens (Author), Rama Gangula (Author), Weiping Yuan (Author), David W. Piston (Author), Mark A. Magnuson (Author)
• Hōputu: Pukapuka
• I whakaputaina: The Company of Biologists, 2011-07-01T00:00:00Z.
• Ngā marau: article
• Urunga tuihono: Connect to this object online.

SUMMARY Fluorescent proteins (FPs) have great utility in identifying specific cell populations and in studying cellular dynamics in the mouse. To quantify the factors that determine both the expression and relative brightness of FPs in mouse embryonic stem cells (mESCs) and in mice, we generated eig...