Reduced off-target effect of NG-BE4max by using NG-HiFi system

• 主要な著者: Huanhuan Shan (著者), Zhiquan Liu (著者), Yingqi Jia (著者), Siyu Chen (著者), Mao Chen (著者), Yuning Song (著者), Tingting Sui (著者), Liangxue Lai (著者), Zhanjun Li (著者)
• フォーマット: 図書
• 出版事項: Elsevier, 2021-09-01T00:00:00Z.
• 主題: article
• オンライン･アクセス: Connect to this object online.

Recently, a rationally engineered SpCas9 variant (SpCas9-NG) that can recognize a minimal NG protospacer adjacent motif (PAM) was reported to expand the targeting scope in genome editing. However, increased genome-wide off-target mutations with this variant compared with SpCas9 were reported in prev...