Reduced off-target effect of NG-BE4max by using NG-HiFi system

• Main Authors: Huanhuan Shan (Author), Zhiquan Liu (Author), Yingqi Jia (Author), Siyu Chen (Author), Mao Chen (Author), Yuning Song (Author), Tingting Sui (Author), Liangxue Lai (Author), Zhanjun Li (Author)
• Format: Book
• Published: Elsevier, 2021-09-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Recently, a rationally engineered SpCas9 variant (SpCas9-NG) that can recognize a minimal NG protospacer adjacent motif (PAM) was reported to expand the targeting scope in genome editing. However, increased genome-wide off-target mutations with this variant compared with SpCas9 were reported in prev...