Reduced off-target effect of NG-BE4max by using NG-HiFi system

• Glavni autori: Huanhuan Shan (Autor), Zhiquan Liu (Autor), Yingqi Jia (Autor), Siyu Chen (Autor), Mao Chen (Autor), Yuning Song (Autor), Tingting Sui (Autor), Liangxue Lai (Autor), Zhanjun Li (Autor)
• Format: Knjiga
• Izdano: Elsevier, 2021-09-01T00:00:00Z.
• Teme: article
• Online pristup: Connect to this object online.

Recently, a rationally engineered SpCas9 variant (SpCas9-NG) that can recognize a minimal NG protospacer adjacent motif (PAM) was reported to expand the targeting scope in genome editing. However, increased genome-wide off-target mutations with this variant compared with SpCas9 were reported in prev...