SPLICELECT™: an adaptable cell surface display technology based on alternative splicing allowing the qualitative and quantitative prediction of secreted product at a single-cell level

• Autores principales: Christel Aebischer-Gumy (Autor), Pierre Moretti (Autor), Romain Ollier (Autor), Christelle Ries Fecourt (Autor), François Rousseau (Autor), Martin Bertschinger (Autor)
• Formato: Libro
• Publicado: Taylor & Francis Group, 2020-01-01T00:00:00Z.
• Materias: article
• Acceso en línea: Connect to this object online.

We describe a mammalian expression construct (SPLICELECT™) that allows the redirection of a proportion of a secreted protein onto the cell surface using alternative splicing: whereas the majority of the RNA is spliced into a transcript encoding a secreted protein, a weak splice donor site yields a s...