SPLICELECT™: an adaptable cell surface display technology based on alternative splicing allowing the qualitative and quantitative prediction of secreted product at a single-cell level

• Asıl Yazarlar: Christel Aebischer-Gumy (Yazar), Pierre Moretti (Yazar), Romain Ollier (Yazar), Christelle Ries Fecourt (Yazar), François Rousseau (Yazar), Martin Bertschinger (Yazar)
• Materyal Türü: Kitap
• Baskı/Yayın Bilgisi: Taylor & Francis Group, 2020-01-01T00:00:00Z.
• Konular: article
• Online Erişim: Connect to this object online.

We describe a mammalian expression construct (SPLICELECT™) that allows the redirection of a proportion of a secreted protein onto the cell surface using alternative splicing: whereas the majority of the RNA is spliced into a transcript encoding a secreted protein, a weak splice donor site yields a s...