Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection-Protocol Optimization and Pitfalls

• Main Authors: Ksenija Vujacic-Mirski (Author), Matthias Oelze (Author), Ivana Kuntic (Author), Marin Kuntic (Author), Sanela Kalinovic (Author), Huige Li (Author), Jacek Zielonka (Author), Thomas Münzel (Author), Andreas Daiber (Author)
• Format: Book
• Published: MDPI AG, 2022-06-01T00:00:00Z.
• Subjects: article
• Online Access: Connect to this object online.

Tetrahydrobiopterin (BH4) is an essential cofactor of all nitric oxide synthase isoforms, thus determination of BH4 levels can provide important mechanistic insight into diseases. We established a protocol for high-performance liquid chromatography/electrochemical detection (HPLC/ECD)-based determin...