Measurement of Tetrahydrobiopterin in Animal Tissue Samples by HPLC with Electrochemical Detection-Protocol Optimization and Pitfalls

• मुख्य लेखकों: Ksenija Vujacic-Mirski (लेखक), Matthias Oelze (लेखक), Ivana Kuntic (लेखक), Marin Kuntic (लेखक), Sanela Kalinovic (लेखक), Huige Li (लेखक), Jacek Zielonka (लेखक), Thomas Münzel (लेखक), Andreas Daiber (लेखक)
• स्वरूप: पुस्तक
• प्रकाशित: MDPI AG, 2022-06-01T00:00:00Z.
• विषय: article
• ऑनलाइन पहुंच: Connect to this object online.

Tetrahydrobiopterin (BH4) is an essential cofactor of all nitric oxide synthase isoforms, thus determination of BH4 levels can provide important mechanistic insight into diseases. We established a protocol for high-performance liquid chromatography/electrochemical detection (HPLC/ECD)-based determin...