Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation

Gene-modified animals, including pigs, can be generated efficiently by introducing CRISPR associated protein 9 (CRISPR/Cas9) into zygotes. However, in many cases, these zygotes tend to become mosaic mutants with various different mutant cell types, making it difficult to analyze the phenotype of gen...

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Main Authors: Shiro YAMASHITA (Author), Yuhei KOGASAKA (Author), Yuuki HIRADATE (Author), Kentaro TANEMURA (Author), Yutaka SENDAI (Author)
Format: Book
Published: The Society for Reproduction and Development, 2019-11-01T00:00:00Z.
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