Suppression of mosaic mutation by co-delivery of CRISPR associated protein 9 and three-prime repair exonuclease 2 into porcine zygotes via electroporation

• Huvudskapare: Shiro YAMASHITA (Författare, medförfattare), Yuhei KOGASAKA (Författare, medförfattare), Yuuki HIRADATE (Författare, medförfattare), Kentaro TANEMURA (Författare, medförfattare), Yutaka SENDAI (Författare, medförfattare)
• Materialtyp: Bok
• Publicerad: The Society for Reproduction and Development, 2019-11-01T00:00:00Z.
• Ämnen: article
• Länkar: Connect to this object online.

Gene-modified animals, including pigs, can be generated efficiently by introducing CRISPR associated protein 9 (CRISPR/Cas9) into zygotes. However, in many cases, these zygotes tend to become mosaic mutants with various different mutant cell types, making it difficult to analyze the phenotype of gen...